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KMID : 0361020000430070746
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Korean Journal of Otolaryngology - Head and Neck Surgery
2000 Volume.43 No. 7 p.746 ~ p.750
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Loss of Heterozygosity on Chromosome 3p, 9p, 17p in Oral & Oropharyngeal Squamous Cell Carcinoma
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Lee Shi-Hyung
Kim Min-Sik
Sun Dong-Il
Song Chi-Joon
Cho Seung-Ho
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Abstract
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Background and Objectives : Patients of oral and oropharyngeal squamous cell carcinoma in the
comparable stage have diverse clinical courses and responses to similar treatment. Understanding the
genetic alterations that occur in these neoplasms may help us understand their biology and behavior. Loss
of heterozygosity(LOH) correlates with inactivated tumor suppressor genes, and tumor suppressor genes
are important in the development of oral and oropharyngeal squamous cell carcinoma. We invested LOH
for the p53, p15, FHIT gene loci by PCR using microsatellite markers.
Materials and Methods : Specimens from 25 patients with oral and oropharyngeal sqamous cell carcinomas were microdissected, and DNA was obtained from normal and tumoral tissues from paraffin embedded sections. PCR analysis was done with three primers, TP53 (17p13.1) for p53 gene and D9S165 (9p21) for p16 gene and D2D1067 (3p21.1-3p14.2)
for FHIT gene. LOH was defined as reduction in the allelic ratio over 50%.
Results : LOH at the p53 locus was found 12 6/17 of the informative cases(35%), and for the p16 locus,
LOH was found in 4/13 of the informative cases (31%). For FHIT locus, LOH was found in 4/15 of the informative
cases (27%).
Conclusion : LOH on chromosome 3 (FHIT gene), Chromosome 9 (p16 gene) and chromosome 17 (p53
gene) occurs in oral and oropharyngeal squamous cell carcinoma with high frequency suggesting that
these tumor suppressor genes are involved in the development and progression of these carcinomas. There
was no correlation between genetic alterations, age, pathologic stage, depth of invasion, cervical lymph
node metastasis, recurrence and differentiation.
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KEYWORD
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Oral cavity, Squamous cell carcinoma, Loss of heterozygosity, p16, p53, FHIT
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